MEDIACLAVE – Media Preparator

Media preparation – fast, reproducible and safe.

The MEDIACLAVE product range allows the rapid and gentle preparation of 1 – 30 L culture medium. Precise controlling and monitoring of temperature, time and pressure during the sterilization process guarantee constant high quality. The intuitive graphical user interface and the simple programming make it very easy for everyone to operate the MEDIACLAVE.

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מק"ט MEDIACLAVE קטגוריה תגית

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Main features 

Hmediaclave_new_2igh Operational Safety
An automatic tightness check at the beginning of each sterilization cycle prevents the instrument from starting if the system is not tight – avoiding incomplete sterilization and subsequent media rejection. Furthermore, MEDIACLAVE is equipped with several independent monitoring systems for pressure and temperature, guaranteeing highest safety standards for the user and the working environment. The vessel lid is equipped with an autonomous overpressure safety valve and a burst disc in case all other electronic monitoring systems fail.

Fast Heating
Powerful heating elements permit fast media processing. This minimises thermal stress and ensures high fertility of your culture medium. Pressure and temperature controlled deaeration guarantees saturated steam in the vessel.

Reliable Sterilisation
The largemediaclave_new_7 and strong magnetic stirrer with adjustable speed and reversing rotation direction ensures homogenous media preparation over a wide viscosity range. A Pt-1000 temperature probe and microprocessor control of process parameters allow reproducible and complete sterilization of culture media.

Rapid Cooling
Rapid coolinmediaclave_new_8g is enabled by an efficient plate heat exchanger. The separation of the cooling water system and the sterilization chamber makes it unlikely that culture media gets contaminated by cooling water. The built-in support pressure system prevents a delay in boiling during the rapid cool down phase, allowing an extraordinary fast and gentle cooling.

System block diagram

1mediaclave_new_6a Media cuvette
2 Sterilisation vessel
3 Temperature probe
4 Adding port
5 Overpressure safety valve
6 Dispense port with decanting tubing
7 Lid seal
8 Magnetic stirrer bar
9 Sterile filter
10 Support pressure pump
11 Flow heater
12 Deaeration outlet
13 Air inlet
14 Quick connection for cooling water inlet
15 Heat exchanger
16 Quick connection for water outlet
17 Quick connection for coupling water
inlet (MEDIACLAVE 30 only)
18 Circulation pump
ValveValve

MEDIACLAVE guarantees gentle and rapid sterilization of standard and highly sensitive culture media. Efficient heating, cooling and homogenous mixing minimize thermal stress and guarantee maximum medium fertility.

Operation modes

Mmediaclave_new_4EDIACLAVE is quickly set up. Insert the cuvette (container for media), fill in the coupling water (water jacket between the cuvette and the vessel for efficient heat transfer) and you are ready to prepare your culture medium.

Culture medium can be suspended and dissolved directly within the MEDIACLAVE. The strong magnetic stirrer guarantees homogeneous mixing within the vessel and prevents coagulation. Alternatively, culture medium can be dissolved and pre-swelled using the WATER BATH operation mode prior to sterilization.

The intuitive, multilingual graphical user interface makes it very easy for everyone to use MEDIACLAVE – no special training of the staff is required.

Up to 50 programs with user-defined parameter settings such as sterilization temperature, sterilization time or dispensing temperature
can be saved and recalled.

Operation modes:

Two main operation modes for media preparation, plus two extra modes can be selected and the parameters set to your needs:

1. STANDARD mode: For the preparation of standard and highly sensitive culture media. Sterilization temperature/time and dispensing temperature can be set.

2. CHOCOLATE AGAR mode: A special two-step program that allows the preparation of complex media. After the first sterilization phase, supplement, e.g. blood, can be added through the adding port. Subsequently, a second heating phase is performed.

3. WATER BATH mode: For pre-swelling culture media prior to sterilization within the temperature range 30 – 80 °C. In combination with an autoclave cuvette (only MEDIACLAVE 10), liquids in glassware can be thermostatted.

4. AUTOCLAVE mode (MEDIACLAVE 10 only): MEDIACLAVE 10 can be used as a benchtop autoclave to sterilize culture medium in glassware such as Erlenmeyer flasks or test tubes.

Standard mode: Heating up, sterilization and cooling down to dispensing temperature.

mediaclave_new_5a

Chocolate Agar mode: After the first sterilization phase, blood is added and the medium heated up again

mediaclave_new_5b

Dispensing

The wide adding port facilitates safe addition of supplements such as antibiotics or blood prior to dispensing. The automatic in-process sterilization of the dispense port minimizes the risk of medium contamination during dispensing.

The vessel and the dispensing system of the MEDIACLAVE were designed to avoid dead volume and to have maximum medium yield.

MEDIACLAVE can be quickly and conveniently connected to the dispensing tubing of the automated Petri dish filler MEDIAJET. Up to 540 Petri dishes can be filled with Agar medium at the touch of a button.

Alternatively, the peristaltic pump DOSE IT can be used to fill containers of more unusual volumes or shapes, e.g. quadrangular Petri dishes, bottles or flasks.

mediaclave_new_10Large containers such as bottles can be filled by pressure dispensing. Medium is dispensed by automatically building up pressure inside the sterilization chamber. Requires optionally available Pressure Dispense Kit.

Process documentation and validation

MEDIACLAmediaclave_new_11VE supports individual needs in process documentation and validation. The sterilization process can be monitored and stored using a PC. A digital signature according to FDA (21 CFR Part 11) / EU (GMP Annex 11) is automatically added to the electronic process file.

All logfiles can also be stored using the MEDIACLAVE USB port.

Imediaclave_new_12f equipped with an optionally available dot matrix printer, high quality printouts insusceptible to fading can be archived. To minimize paper consumption, the printout intervals can be increased

Capacity MEDIACLAVE 10 MEDIACLAVE 30
Stainless steel cuvette 1 – 10 litres 3 – 30 litres
Autoclave cuvette (Ø, H) 254 mm, 203 mm
Temperature range
Sterilisation 30 °C – 122 °C 30 °C – 122 °C
Dispensing 25 °C – 80 °C 25 °C – 80 °C
Water bath 30 °C – 80 °C 30 °C – 80 °C
Max. temperature deviation +1.0 °C/-0.2 °C +1.0 °C/-0.2 °C
Time
Heating up (25 °C – 121 °C) 20 – 60 min 20 – 60 min
Sterilisation 1 – 99 min 1 – 99 min
Cooling down (121°C – 55°C) 5 – 20 min 5 – 30 min
Stirrer speed
Selectable 50 – 200 rpm, reversing direction 50 – 200 rpm, reversing direction
Utilities
Heating Capacity Max. 3 kW Max. 9 kW
Interfaces 2 x RS232, Ethernet, USB port, 2 x RS232, Ethernet, USB port
AUX contact, footswitch, AUX contact, footswitch,
ext. pinch valve ext. pinch valve
Cooling water connections ¾” thread ¾” thread
Graphic LCD-display 240 x 128 points 240 x 128 points
Electricity supply
All MEDIACLAVE 10 instruments 200 – 240 V 50/60 Hz, 16 A
MEDIACLAVE 30, US/JP (136 050) 200 – 208 V 3~/PE, 50/60 Hz, 30 A
MEDIACLAVE 30, EU (136 055) 400 V 3~/N/PE, 50/60 Hz, 16 A
Dimensions and weight
Basic Device (H x W x D) 480 mm x 550 mm x 640 mm 1040 mm x 550 mm x 640 mm
Weight 57 kg 85 kg
השאירו לנו הודעה

שירות טכני: 08-683-6306
מכירות: 072-395-0508
מייל: info@medent.co.il

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